Anti-PTEN antibody [4D8]

Anti-PTEN antibody [4D8]

• 概述

• 产品描述As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23.31. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers.PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells.
• 产品名称Anti-PTEN antibody [4D8]
• 分子量55kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus
• 其它名称
  • 10q23del antibody
  • BZS antibody
  • DEC antibody
  more

• 应用

  WB: 1:1,000
  IP: 1-2 μg per 100-500 μg of total protein
  IF: 1:50-500

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  Fig1: Western blot analysis of PTEN expression in A-431 (A) and KNRK (B) whole cell lysates.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！