Anti-CK II alpha antibody

Anti-CK II alpha antibody

• 概述

• 产品描述Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation.

• 产品名称Anti-CK II alpha antibody

• 分子量45 KDa

• 种属反应性Human,Mouse,Rat,Dog,Cow

• 验证应用WB

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CK II alpha 201-300/391

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

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• ​其它名称STK
  Casein kinase II Alpha
  CKII alpha
  Serine/Threonine Protein Kinase II Alpha
  CKⅡalpha
  Casein kinase 2 alpha 1 polypeptide
  Casein kinase II alpha 1 subunit
  Casein kinase II alpha chain
  Casein kinase II alpha subunit
  Casein kinase II subunit alpha
  Casein kinase II alpha 1
  CK II alpha
  CKIIalpha
  CSK21_HUMAN
  CK II
  CK2 alpha
  CK2 catalytic subunit alpha
  CK2A1
  CKII
  CSNK2A1.

  more

• 应用

  WB:1:500-2000

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  Fig1: Sample: A431 Cell Lysate at 30 ug
  Primary: Anti- CK II alpha at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45 kD
  Observed band size: 45 kD

   

  

  Fig2: Sample: HepG2 Cell Lysate at 30 ug
  Primary: Anti-CK II alpha at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45 kD
  Observed band size: 45 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！