Anti-HERC6 antibody

Anti-HERC6 antibody

• 概述

• 产品描述E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity).

• 产品名称Anti-HERC6 antibody

• 分子量115 KDa

• 种属反应性Human,Mouse,Rat,Pig,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human HERC6 751-850/1022

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm, cytosol.

•  

• ​其它名称EC 6.3.2.
  FLJ20637
  HECT domain and RCC1 like domain containing protein 6
  HECT domain and RCC1-like domain-containing protein 6
  Hect domain and RLD 6
  HERC 6
  HERC6
  HERC6_HUMAN
  Potential ubiquitin ligase
  Probable E3 ubiquitin protein ligase HERC6
  Probable E3 ubiquitin-protein ligase HERC6.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Embryo (Mouse) Lysate at 40 ug
  Primary: Anti-HERC6  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 115 kD
  Observed band size: 115 kD

   

  

  Fig2: Sample:
  Testis (Mouse) Lysate at 40 ug
  Primary: Anti-HERC6  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 115 kD
  Observed band size: 115 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample:Brain (Mouse) Lysate at 40 ug
  Primary: Anti-HERC6(at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 115kD
  Observed band size: 115kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！