Anti-SEMA4D antibody

Anti-SEMA4D antibody

• 概述

• 产品名称Anti-SEMA4D antibody
• 分子量60, 126 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human SEMA4D 75-170/862
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane.
• 其它名称A8 antibody BB 18
  BB18
  CD 100
  CD100
  CD100 antigen
  Coll 4
  COLL4
  Collapsin 4
  Collapsin4
  GR3
  Human semaphorin CD100 mRNA complete cds
  Leukocyte activation antigen CD100
  M sema G
  MSEMA
  SEM4D_HUMAN
  Sema 4d
  Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D
  Sema H
  SEMA J
  SEMA4D
  Semacl 2
  Semacl2
  SemaH
  SEMAJ
  Semaphorin 4D
  Semaphorin 4D precursor
  Semaphorin C like 2
  Semaphorin H
  Semaphorin J
  Semaphorin-4D
  Semaphorin4D
  SemaphorinJ
  Semcl 2
  Semcl2.
  more

• 应用

  WB:1:500-2000
  FC:1μg /Test

•  

  

  Fig1: Blank control: Raji(blue).
  Primary Antibody:Rabbit Anti-SEMA4D antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1916-36, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Muscle (Mouse) Lysate at 40 ug
  Primary: Anti-SEMA4Dat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60/126 kD
  Observed band size: 60 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！