Anti-GTPBP9 antibody

Anti-GTPBP9 antibody

• 概述

• 产品描述Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.

• 产品名称Anti-GTPBP9 antibody

• 分子量45 KDa

• 种属反应性Human,Mouse,Rat,Dog,Cow,Sheep,Fruit Fly

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GTPBP9 151-250/396

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Predominantly cytoplasmic, shuttles between the nucleus and the cytoplasm.

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• ​其它名称DNA damage-regulated overexpressed in cancer 45 protein
  DOC45
  GBP45
  GTP-binding protein 9 (putative)
  GTP-binding protein 9
  GTP-binding protein PTD004
  homologous yeast-44.2 protein
  Obg like ATPase 1
  Obg-like ATPase 1
  OLA1
  OLA1_HUMAN
  PTD004.

  more

• 应用

  WB:1:500-2000
  FC:1ug/Test

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  Fig1: Sample:
  HepG2 Cell (Human) Lysate at 30 ug
  TT Cell (Human) Lysate at 30 ug
  Primary: Anti-GTPBP9  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45kD
  Observed band size: 50kD

   

  

  Fig2: Sample: Raji Cell (Human) Lysate at 30 ug
  Primary: Anti-GTPBP9  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45kD
  Observed band size: 50kD

   

  

  Fig3: Sample: Brain (Mouse) Lysate at 40 ug
  Primary: Anti-GTPBP9  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45kD
  Observed band size: 50kD

   

  

  Fig4: Blank control (Black line):Molt4 (Black).
  Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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