Anti-NARG1 antibody

Anti-NARG1 antibody

• 概述

• 产品描述Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
• 产品名称Anti-NARG1 antibody
• 分子量101 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human NARG1 121-220/866
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
• 其它名称5730450D16Rik
  6330400I15
  ASTBDN
  GA19
  Gastric cancer antigen Ga19
  MGC29428
  mNAT1
  N alpha acetyltransferase 15, NatA auxiliary subunit
  N terminal acetyltransferase
  N(alpha) acetyltransferase 15, NatA auxiliary subunit
  N-alpha-acetyltransferase 15
  N-terminal acetyltransferase
  Naa15
  NAA15_HUMAN
  NatA auxiliary subunit
  NATH
  NMDA receptor regulated 1
  NMDA receptor regulated protein 1
  NMDA receptor-regulated protein 1
  Protein tubedown 1
  Protein tubedown-1
  Tbdn 1
  TBDN100
  Transcriptional coactivator tubedown 100
  tubedown-1.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  SP2/0 (Mouse) Lysate at 30 ug
  Primary: Anti-NARG1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 101 kD
  Observed band size: 101 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugated) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NARG1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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