Anti-NPRC antibody

Anti-NPRC antibody

• 概述

• 产品描述Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
• 产品名称Anti-NPRC antibody
• 分子量57 KDa
• 种属反应性Human,Mouse,Rat,Cow,Horse
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human NPR-C 301-400/541
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Single-pass type I membrane protein.
• 其它名称ANP C
  ANPC
  ANPRC
  Atrial natriuretic peptide C type receptor
  Atrionatriuretic peptide receptor C
  Natriuretic peptide receptor C/guanylate cyclase C
  NPR 3
  NPR C
  NPR3
  NPRC
  ANPRC_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  HL-60 (Human) Cell Lysate at 40 ug
  A431 (Human) Cell Lysate at 40 ug
  Kidney (Mouse) Lysate at 40 ug
  Primary: Anti-NPRC at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 57 kD
  Observed band size: 57 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (NPRC) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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