Anti-ODC antibody
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概述
- 产品描述SLC25A21 is a homolog of the S. cerevisiae ODC proteins, mitochondrial carriers that transport C5-C7 oxodicarboxylates across inner mitochondrial membranes. One of the species transported by ODC is 2-oxoadipate, a common intermediate in the catabolism of lysine, tryptophan, and hydroxylysine in mammals. Within mitochondria, 2-oxoadipate is converted into acetyl-CoA.
- 产品名称Anti-ODC antibody
- 分子量51 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ODC 321-461/461
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Mitochondrion inner membrane
- 其它名称Ornithine decarboxylase 1
Ornithine decarboxylase
Dodc1
Odc 1
ODC
Odc1
ODC2
Ornithine decarboxylase 2
DCOR_HUMAN
Ornithine decarboxylase 1
Ornithine decarboxylase
Ornithine decarboxylase structural 1
RNODC.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC( at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Fig3: Sample:Liver(Mouse) lysate at 30ug;
Primary: Anti-ODC(ER1914-29) at 1:300 dilution;
Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution;
Predicted band size : 51kD
Observed band size : 51kD
Fig4: Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-OD at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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