Anti-PAFAH antibody

Anti-PAFAH antibody

• 概述

• 产品描述Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

• 产品名称Anti-PAFAH antibody

• 分子量48 KDa

• 种属反应性Human,Mouse,Rat,Dog

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PAFAH 301-441/441

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted, extracellular space.

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• ​其它名称Lp-PLA2
  PLA2G7
  PAFAH
  lipoprotein-associated phospholipase A2
  Platelet-activating factor acetylhydrolase
  PAF acetylhydrolase
  PAF 2-acylhydrolase
  LDL-associated phospholipase A2
  LDL-PLA(2)
  2-acetyl-1-alkylglycerophosphocholine esterase
  1-alkyl-2-acetylglycerophosphocholine esterase
  PAFA_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Lung (Rat) Lysate at 40 ug
  Primary: Anti-PAFAH  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 48 kD

   

  

  Fig3: Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated(ER1914-69) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated  at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining

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