Anti-PKM2 antibody
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概述
- 产品描述In mammals, four different isoenzymes exist for pyruvate kinase. Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
- 产品名称Anti-PKM2 antibody
- 分子量58 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human PKM2 aa 380-430.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称CTHBP antibody
Cytosolic thyroid hormone binding protein antibody
Cytosolic thyroid hormone-binding protein antibody
KPYM_HUMAN antibody
MGC3932 antibody
OIP 3 antibody
OIP-3 antibody
OIP3 antibody
OPA interacting protein 3 antibody
Opa-interacting protein 3 antibody
p58 antibody
PK muscle type antibody
PK, muscle type antibody
PK2 antibody
PK3 antibody
PKM antibody
PKM2 antibody
pykm antibody
Pyruvate kinase 2/3 antibody
Pyruvate kinase 3 antibody
Pyruvate kinase isozymes M1/M2 antibody
Pyruvate kinase muscle antibody
Pyruvate kinase muscle isozyme antibody
pyruvate kinase PKM antibody
Pyruvate kinase, muscle 2 antibody
TCB antibody
THBP1 antibody
Thyroid hormone binding protein 1 antibody
Thyroid hormone binding protein cytosolic antibody
Thyroid hormone-binding protein 1 antibody
Tumor M2 PK antibody
Tumor M2-PK antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of PKM2 on 293 cell lysate using anti-PKM2 antibody at 1/500 dilution.
Fig2: ICC staining PKM2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PKM2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PKM2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
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