Anti-PPP2R5E antibody

Anti-PPP2R5E antibody

• 概述

• 产品描述The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

• 产品名称Anti-PPP2R5E antibody

• 分子量55 KDa

• 种属反应性Human,Mouse,Rat,Horse

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PPP2R5E 121-220/467

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm.

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• ​其它名称2A5E_HUMAN
  Epsilon isoform of regulatory subunit B56 protein phosphatase 2A
  PP2A B subunit B' epsilon
  PP2A B subunit B' epsilon isoform
  PP2A B subunit B56 epsilon
  PP2A B subunit B56 epsilon isoform
  PP2A B subunit isoform B'-epsilon
  PP2A B subunit isoform B'-epsilon
  PP2A B subunit isoform B56-epsilon
  PP2A B subunit isoform PR61-epsilon
  PP2A B subunit isoform R5-epsilon
  PP2A B subunit PR61 epsilon
  PP2A B subunit PR61 epsilon isoform
  PP2A B subunit R5 epsilon
  PP2A B subunit R5 epsilon isoform
  PPP2R5E
  Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform
  Protein phosphatase 2 regulatory subunit B' epsilon isoform
  Regulatory subunit B of protein phosphatase 2 epsilon isoform
  Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:
  Testis (Mouse) Lysate at 40 ug
  Primary: Anti- PPP2R5E at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

   

  

  Fig2: Sample:
  Thymus (Mouse) Lysate at 40 ug
  Primary: Anti- PPP2R5E at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

   

  

  Fig4: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Epithelium (Mouse) Lysate at 40 ug
  Primary: Anti-PPP2R5Eat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55kD
  Observed band size: 55kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！