Anti-CRK antibody [C1-G11]

Anti-CRK antibody [C1-G11]

• 概述

• 产品描述The Crk family of adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities . Crk-II has less transforming activity than Crk-I, although both Crk-I and Crk-II bind to many tyrosine-phosphorylated proteins that bind to grb2. In addition, Crk-II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.
• 产品名称Anti-CRK antibody [C1-G11]
• 分子量38 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytooplasm. Cell membrane.
• 其它名称
  • Adapter molecule crk antibody
  • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
  • CRK antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CRK on human CRK recombinant protein using anti-CRK antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CRK on HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate using anti-CRK antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining CRK (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of MCF-7 cells with CRK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

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