Anti-His-Tag antibody
-
概述
- 产品描述His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- 产品名称Anti-His-Tag antibody
- 验证应用WB
- 抗体类型兔多抗
- 免疫原Synthetic peptide immune sequence is HHHHHHC.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
-
-
Fig1: Western blot analysis of His-Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
