Anti-RNF128 antibody

Anti-RNF128 antibody

• 概述

• 产品描述E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
• 产品名称Anti-RNF128 antibody
• 分子量43 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Zebrafish
• 验证应用WB,IHC-P,ICC/IF
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human RNF128 151-260/428
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Endomembrane system; Single-pass membrane protein. Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctuate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the e
• 其它名称E3 ubiquitin protein ligase RNF128
  E3 ubiquitin-protein ligase RNF128
  FLJ23516
  Gene related to anergy in lymphocytes protein
  GRAIL
  ring finger protein 128
  RN128_HUMAN
  RNF128.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:50-200

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RNF128) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Kidney (Mouse) Lysate at 40 ug
  293T(Human) Cell Lysate at 30 ug
  Ovary (Mouse) Lysate at 40 ug
  Primary: Anti- RNF128at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 62 kD

   

  

  Fig5: Sample:
  293T(Human) Cell Lysate at 30 ug
  Primary: Anti- RNF128  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 61kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！