Anti-MGMT antibody

Anti-MGMT antibody

• 概述

• 产品描述MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.

• 产品名称Anti-MGMT antibody

• 分子量22 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Nucleus

•  

• 其它名称6 O methylguanine DNA methyltransferase antibody
  6-O-methylguanine-DNA methyltransferase antibody
  Agat antibody
  AGT antibody
  AI267024 antibody
  EC 2.1.1.63 antibody
  Methylated DNA protein cysteine methyltransferase antibody
  Methylated-DNA--protein-cysteine methyltransferase antibody
  Methylguanine DNA methyltransferase antibody
  MGC107020 antibody
  MGMT antibody
  MGMT_HUMAN antibody
  O 6 methylguanine DNA alkyltransferase antibody
  O 6 methylguanine DNA methyltransferase antibody
  O-6-methylguanine-DNA methyltransferase antibody
  O-6-methylguanine-DNA-alkyltransferase antibody

  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC:1:100

•  

  

  Fig1: Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: MCF-7
  Lane 3: Daudi
  Lane 4: Raji
  Lane 5: SK-BR-3
  Lane 6: SKOV3
  Lane 7: Human liver
  Lane 8: Human kidney
  Lane 9: Human thymus
  Lane 10: Human placentae

   

  

  Fig2: ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin.

•  

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！