Anti-HLA-DOα antibody
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概述
- 产品描述HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level.
- 产品名称Anti-HLA-DOα antibody
- 分子量34 kDa
- 种属反应性Human
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human HLA-DOA. AA range:71-120
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称HLA-DOA antibody; HLA-DNA antibody; HLA-DZA antibody; HLA class II histocompatibility antigen antibody; DO alpha chain antibody; MHC DN-alpha antibody; MHC DZ alpha antibody; MHC class II antigen DOA antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of various cells using HLA-DOα Polyclonal Antibody
Fig2: Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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