Anti-Tyrosinase antibody

Anti-Tyrosinase antibody

• 概述

• 产品描述This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
• 产品名称Anti-Tyrosinase antibody
• 分子量70 kDa

• 种属反应性Human,Mouse

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Membrane.

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• 其它名称

  more

  • ATN antibody

  • CMM8 antibody

  • LB24 AB antibody

• 应用

  WB: 1:200-1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC:1:50-1:200

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  Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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