Anti-OTUB2 antibody
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概述
- 产品描述Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. In the structure described by PubMed:15258613, the Asp-48 active site of the catalytic triad is located too far to interact directly with the active site His-224. A possible explanation is that OTUB2 is in inactive conformation in absence of ubiquitin and a conformation change may move Asp-48 in the proximity of His-224 in presence of ubiquitin substrate. Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
- 产品名称Anti-OTUB2 antibody
- 分子量27 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human OTUB2. AA range:133-182
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 亚细胞定位Nucleus.
- 其它名称
- OTUB2 antibody
- C14orf137 antibody
- OTB2 antibody
more
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western blot analysis of lysates from COLO, HepG2, Jurkat, 293, and HT-29 cells, using OTUB2 Antibody. The lane on the right is blocked with the synthesized peptide.
Fig2: Western Blot analysis of various cells using OTUB2 Polyclonal Antibody diluted at 1:2000
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!