Anti-Phospholipase C beta 2 antibody

Anti-Phospholipase C beta 2 antibody

• 概述

• 产品描述The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

• 产品名称Anti-Phospholipase C beta 2 antibody

• 分子量130 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P,ICC/IF,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Phospholipase C beta 2 401-500/1185

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

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• ​其它名称PLCβ2
  1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2
  1-phosphatidylinositol-4
  5-bisphosphate phosphodiesterase beta-2
  FLJ38135
  Phosphoinositide phospholipase C-beta-2
  Phospholipase C-beta-2
  PLC-beta-2
  Plcb2
  PLCB2_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test
  IF:1:50-200

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample:
  K562(Human) Cell Lysate at 30 ug
  Primary: Anti-Phospholipase C beta 2 ( at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 130 kD
  Observed band size: 130 kD

   

  

  Fig5: Blank control: Hela Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
  Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody  Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

   

  

  Fig6: Sample:
  HL-60(Human) Cell Lysate at 30 ug
  Primary: Anti- Phospholipase C beta 2 ( at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 130 kD
  Observed band size: 130 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！