Anti-Smad2/3 antibody
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概述
- 产品描述The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation
- 产品名称Anti-Smad2/3 antibody
- 分子量48 kDa
- 种属反应性Human, Mouse, Rat, Monkey
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human Smad2/3. AA range:1-50
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- SMAD2 antibody; MADH2 antibody; MADR2 antibody; Mothers against decapentaplegic homolog 2 antibody; MAD homolog 2 antibody; Mothers against DPP homolog 2 antibody; JV18-1 antibody; Mad-related protein 2 antibody; hMAD-2 antibody; SMAD family member 2 antibody; SMAD 2 antibody; Smad2 antibody; hSMAD2 antibody; SMAD3 antibody; MADH3 antibody; Mothers against decapentaplegic antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,Smad2/3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Fig2: Western Blot analysis of various cells using Smad2/3 Polyclonal Antibody diluted at 1：500
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