Anti-IDH3A antibody
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概述
- 产品描述Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
- 产品名称Anti-IDH3A antibody
- 分子量39 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原Synthesized peptide derived from the Internal region of human IDH3A.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- IDH3A antibody; Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial antibody; Isocitric dehydrogenase subunit alpha antibody; NAD(+)-specific ICDH subunit alpha antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of MCF-7, HT29, mouse brain, mouse heart, mouse colon, mouse kidney, mouse skeletal muscle cells using IDH3A Polyclonal Antibody.. Secondary antibody（catalog#：HA1001) was diluted at 1:20000
Fig2: Immunohistochemical analysis of paraffin-embedded human-心, antibody was diluted at 1:100
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