Anti-MRP8/S100A8 antibody

Anti-MRP8/S100A8 antibody

• 概述

• 产品描述The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, Cal-granulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins) and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/ relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in Leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells.
• 产品名称Anti-MRP8/S100A8 antibody
• 分子量11 kDa

• 种属反应性Human, Rat

• 验证应用WB, ICC, IHC-P, FC

• 抗体类型兔多抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein A purified.

• 亚细胞定位Cell membrane. Cytoplasm. Secreted.

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• 其它名称

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• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of MRP8/S100A8 on HL-60 cell lysate using anti-MRP8/S100A8 antibody at 1/100 dilution.

   

  

  Fig2: ICC staining MRP8/S100A8 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining MRP8/S100A8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining MRP8/S100A8 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

   

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MRP8/S100A8 antibody. Counter stained with hematoxylin.

   

  

  Fig10: Flow cytometric analysis of THP-1 cells with MRP8/S100A8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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