Anti-KChIP1 antibody

Anti-KChIP1 antibody

• 概述

• 产品描述This gene encodes a member of the family of cytosolic voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the neuronal calcium sensor (NCS) family of the calcium binding EF-hand proteins. They associate with Kv4 alpha subunits to form native Kv4 channel complexes. The encoded protein may regulate rapidly inactivating (A-type) currents, and hence neuronal membrane excitability, in response to changes in the concentration of intracellular calcium. Alternative splicing results in multiple transcript variants encoding different isoforms.
• 产品名称Anti-KChIP1 antibody
• 分子量Predicted band size 27 kDa.
• 种属反应性Human,Rat
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within rat KChIP1 aa 1-50.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Cell membrane, cytoplasm, dendrite.
• 其它名称
  • A type potassium channel modulatory protein 1 antibody
  • A-type potassium channel modulatory protein 1 antibody
  • KChIP1 antibody
  more

• 应用

  WB:1:500
  IHC-P:1:50-1:100
  FC:1:50-1:100

•  

  

  Fig1: Western blot analysis of KChIP1 on human skin tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-KChIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

   

  

  Fig3: Flow cytometric analysis of KChIP1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody  (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！