Anti-RPA1 antibody [D9-C4]

Anti-RPA1 antibody [D9-C4]

• 概述

• 产品描述RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
• 产品名称Anti-RPA1 antibody [D9-C4]
• 分子量70 kDa
• 种属反应性Human,Monkey
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • Dmrpa1 antibody
  • Drosophila Replication Protein A antibody
  • DRPA antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of RPA70 on human RPA70 recombinant protein using anti-RPA70 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of RPA70 on different cell lysates using anti-RPA70 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: MCF-7
  Lane 3: K562
  Lane 4: A431
  Lane 5: COS-7

   

  

  Fig3: ICC staining RPA70 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-RPA70 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded prostate tissue using anti-RPA70 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Jurkat cells with RPA70 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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