Anti-Phospho-PKA C (Thr198) antibody
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概述
- 产品描述The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. The phosphorylation at Threonine 198 is cAMP dependent.
- 产品名称Anti-Phospho-PKA C (Thr198) antibody
- 分子量PKAα cat: 40 kDa, PKAβ cat: 53 kDa, PKAγ cat: 39-40 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF
- 抗体类型兔多抗
- 免疫原A short amino acid sequence containing Thr 198 phosphorylated PKAα cat of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm, Nucleus, Membrane, Cell membrane
- 其它名称
- cAMP dependent protein kinase catalytic subunit alpha antibody
- cAMP dependent protein kinase catalytic subunit beta antibody
- cAMP dependent protein kinase catalytic subunit gamma antibody
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
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Fig1: Western blot analysis of PKAα cat phosphorylation in non-transfected (A) and human PKAα cat transfected (B) 293T whole cell lysates.
Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
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