Anti-PDGF Receptor alpha antibody
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概述
- 产品描述Platelet derived growth factor (PDGF) is a mitogen for mesenchyme- and gliaderived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ, and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. The genes encoding PDGFR-α and -β map to human chromosome 4q12 and 5q31-32, respectively. Translocation of the PDGFR-β gene with the Tel gene is linked with chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.
- 产品名称Anti-PDGF Receptor alpha antibody
- 分子量170kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cell membrane
- 其它名称
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
FC: 1 μg per 1 x 106 cells
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Fig1: Western blot analysis of PDGFR-α expression in NIH/3T3 whole cell lysates (A,B).
Fig2: Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal mouse skin showing cytoplasmic and membrane staining.
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