Anti-Isocitrate dehydrogenase/IDH1 antibody
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概述
- 产品描述Isocitrate dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and CO2. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step of the citric acid cycle while converting NAD+ to NADH in the mitochondria. The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They localize to the cytosol as well as the mitochondrion and peroxisome. Mutations in IDH1 are also implicated in cancer. Originally mutations in IDH1 were detected in an integrated genomic analysis of human glioblastoma multiforme. In addition to being mutated in diffuse gliomas, IDH1 has also been shown to harbor mutations in human acute myeloid leukemia (AML).
- 产品名称Anti-Isocitrate dehydrogenase/IDH1 antibody
- 分子量47 kDa
- 种属反应性Human,Zebrafish
- 验证应用WB,IHC-P
- 抗体类型小鼠单抗
- 免疫原recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm
- 其它名称
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
more
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应用
WB: 1:1,000
IHC-P: 1:100-1:200
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Fig1: Western blot analysis of IDH1 on different cell lysates using anti-IDH1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: MCF-7
Lane 5: A549
Lane 6: Jurkat
Lane 7: Human kidney
Lane 8: Human brain
Lane 9: Human liver
Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
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