Anti-ENAH antibody
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概述
- 产品描述This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3.
- 产品名称Anti-ENAH antibody
- 分子量Predicted band size: 67 kDa.
- 种属反应性Human,Mouse,Rat
- 验证应用WB
- 抗体类型兔多抗
- 免疫原Synthetic peptide within C-terminal human ENAH.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion.
- 其它名称
- ENA antibody
- Enah antibody
- ENAH_HUMAN antibody
more
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Fig1: Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Fig2: Western blot analysis of ENAH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodywas used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: Rat brain tissue lysate
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