Anti-IRAK4 antibody [B9-H2]

Anti-IRAK4 antibody [B9-H2]

• 概述

• 产品描述Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. IRAK family members include two active kinases, IRAK-1 and IRAK-4, and two inactive kinases, IRAK-2 and IRAK-M. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway and MAP kinase pathways. IRAK-4 appears to act upstream of other IRAKs and phosphorylates IRAK-1 on threonine 387. It is highly expressed in liver and kidney tissues, but also displays a wide, low level of expression in other tissues. IRAK-4 is an essential component of innate immunity. Deficiency of IRAK-4 leads to recurrent bacterial infections and profound hyporesponsiveness to LPS and IL-1. Therefore, IRAK-4 may be a potential target for therapeutic drug design.
• 产品名称Anti-IRAK4 antibody [B9-H2]
• 分子量52 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • IL-1 receptor-associated kinase 4 antibody
  • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
  • Interleukin-1 receptor-associated kinase 4 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of IRAK4 on human IRAK4 recombinant protein using anti-IRAK4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of IRAK4 on different cell lysates using anti-IRAK4 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: THP-1
  Lane 2: Hela
  Lane 3: K562
  Lane 4: MCF-7
  Lane 5: RAW264.7
  Lane 6: Jurkat
  Lane 7: Cos7

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IRAK4 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-IRAK4 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with IRAK4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

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