Anti-SETDB1 antibody [A12-H6]

Anti-SETDB1 antibody [A12-H6]

• 概述

• 产品描述ERG-associated protein with SET domain (ESET), also designated Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily. It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modulation. ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin. The gene encoding for this protein, SETDB1, maps to chromosome 1q21. ESET is a histone methyltransferase, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity. This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones. ESET is widely expressed with highest levels found in testis.
• 产品名称Anti-SETDB1 antibody [A12-H6]
• 分子量180 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • AU022152 antibody
  • EC 2.1.1.43 antibody
  • ERG-associated protein with SET domain antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:1,000

•  

  

  Fig1: Western blot analysis of SETDB1 on different cell lysates using anti-SETDB1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: MCF-7
  Lane 2: T47D
  Lane 3: HEK293
  Lane 4: JURKAT
  Lane 5: NIH/3T3
  Lane 6: F9
  Lane 7: RAW246.7
  Lane 8: Cos7

   

  

  Fig2: ICC staining SETDB1 (green) and actin filaments (red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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