Anti-PPM1A antibody [H6-F12]

Anti-PPM1A antibody [H6-F12]

• 概述

• 产品描述Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
• 产品名称Anti-PPM1A antibody [H6-F12]
• 分子量43 kDa
• 种属反应性Human,Monkey
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol, Nucleus.
• 其它名称
  • Protein phosphatase 2C isoform alpha antibody
  • EC 3.1.3.16 antibody
  • FLJ42306 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PPM1A on human PPM1A recombinant protein using anti-PPM1A antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PPM1A on HEK293 (1) and PPM1A-hIgGFc transfected HEK293 (2) cell lysate using anti-PPM1A antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PPM1A on different lysates using anti-PPM1A antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: Jurkat
  Lane 3: A431
  Lane 4: HeLa
  Lane 5: HEK293
  Lane 6: Raji
  Lane 7: MCF-7
  Lane 8: COS7

   

  

  Fig4: Flow cytometric analysis of Hela cells with PPM1A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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