Anti-PRMT5 antibody [7-C2]

Anti-PRMT5 antibody [7-C2]

• 概述

• 产品描述The formation of the spliceosome includes the assembly of Sm proteins inan ordered manner onto snRNAs. This process is mediated by the survivalof a motor neuron (SMN) protein, and is enhanced by modification of specificArginine residues in the Sm proteins to symmetrical dimethylarginines(sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome,a complex comprised of the type II methyltransferase PRMT5 (alsodesignated JAK-binding protein 1, JBP1), pICln and two novel factors. PRMT5binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, whilepICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes tothe cytoplasm in a wide variety of tissues. PRMT5 also associates specificallywith the transcription start site region of the cyclin E1 promoter and, therefore,is involved in the control of transcription and proliferation.The geneencoding human PRMT5 maps to chromosome 14q11.2.
• 产品名称Anti-PRMT5 antibody [7-C2]
• 分子量72 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, cytoplasm.
• 其它名称
  • 72 kDa ICln binding protein antibody
  • 72 kDa ICln-binding protein antibody
  • ANM5_HUMAN antibody
  more

• 应用

  WB: 1:1,000
  IHC-P: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.

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