Anti-C-CBL antibody [C1-B12]

Anti-C-CBL antibody [C1-B12]

• 概述

• 产品名称Anti-C-CBL antibody [C1-B12]
• 分子量120 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane, Cytoplasm.
• 其它名称
  • 4732447J05Rik antibody
  • C CBL antibody
  • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: RAJI
  Lane 2: RAW264.7
  Lane 3: K562
  Lane 4: SKBR-3
  Lane 5: 3T3-L1
  Lane 6: THP-1
  Lane 7: PC-12

   

  

  Fig3: ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

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