Anti-Grp75 antibody [6-A9]

Anti-Grp75 antibody [6-A9]

• 概述

• 产品描述The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.
• 产品名称Anti-Grp75 antibody [6-A9]
• 分子量74 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion. Nucleus.
• 其它名称
  • 75 kDa glucose regulated protein antibody
  • 75 kDa glucose-regulated protein antibody
  • CSA antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Grp75 on different cell lysates using anti-Grp75 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: NIH-3T3
  Lane 2: HepG2
  Lane 3: K562
  Lane 4: L929

   

  

  Fig2: ICC staining Grp75 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Grp75 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining Grp75 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Grp75 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Grp75 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Grp75 antibody. Counter stained with hematoxylin.

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