Anti-PI3-kinase p85 subunit alpha antibody [L3-D6]

Anti-PI3-kinase p85 subunit alpha antibody [L3-D6]

• 概述

• 产品描述Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.
• 产品名称Anti-PI3-kinase p85 subunit alpha antibody [L3-D6]
• 分子量84 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human PI3-kinase p85 subunit alpha aa 1-200.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cytosol. Endoplasmic reticulum. Golgi apparatus. Nucleus. Plasma Membrane.
• 其它名称
  • GRB1 antibody
  • p85 alpha antibody
  • p85 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of PI3-kinase p85 subunit alpha on Raji (1) and U937 (2) using anti-PI3-kinase p85 subunit alpha antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining PI3-kinase p85 subunit alpha (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining PI3-kinase p85 subunit alpha (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI3-kinase p85 subunit alpha antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PI3-kinase p85 subunit alpha antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PI3-kinase p85 subunit alpha antibody. Counter stained with hematoxylin.

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