Anti-PRDM5 antibody [12C-D4]

Anti-PRDM5 antibody [12C-D4]

• 概述

• 产品描述A cDNA of PRDM5 was isolated based upon its homology to the PR domain of PRDM2. The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Through radiation hybrid analysis, PRDM5 was mapped to human chromosome 4q27, a region thought to contain tumor suppressor genes for ovarian, breast, lung, liver, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. Upon infection of tumor cells, a recombinant adenovirus expressing PRDM5 causes G2/M arrest and apoptosis, suggesting that inhibition of PRDM5 may be involved in carcinogenesis.
• 产品名称Anti-PRDM5 antibody [12C-D4]
• 分子量73 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • BCS2 antibody
  • PFM 2 antibody
  • PFM2 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PRDM5 on human PRDM5 recombinant protein using anti-PRDM5 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PRDM5 on HEK293 (1) and PRDM5-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM5 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PRDM5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Hela cells with PRDM5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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