Anti-RAD50 antibody
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概述
- 产品描述Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad54A of the DEAD-like helicase superfamily binds to double-stranded DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer.
- 产品名称Anti-RAD50 antibody
- 分子量154 kDa
- 种属反应性Human,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2a
- 纯化方式Protein A purified.
- 亚细胞定位Nucleus.
- 其它名称
- DNA repair protein RAD50 antibody
- hRAD50 antibody
- hsRAD50 antibody
more
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:100-1:200
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Fig1: Western blot analysis of RAD50 on human RAD50 recombinant protein using anti-RAD50 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RAD50 on HEK293 (1) and RAD50-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of RAD50 on C6 (1) and HepG2 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution.
Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with RAD50 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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