Anti-EZR antibody [F1-9A]

Anti-EZR antibody [F1-9A]

• 概述

• 产品描述Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins (2-5). Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.
• 产品名称Anti-EZR antibody [F1-9A]
• 分子量70 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Apical cell membrane. Cell projection.
• 其它名称
  • Villin 2 ezrin antibody
  • CVIL antibody
  • CVL antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of EZR on human EZR recombinant protein using anti-EZR antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of EZR on HEK293 (1) and EZR-hIgGFc transfected HEK293 (2) cell lysate using anti-EZR antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of EZR on different cell lysate using anti-EZR antibody at 1/1,000 dilution.
  Positive control: Line1: MCF-7 Line2: Hela Line3: A431 Line4: Hek293 Line5: SK-N-SH Line6: Jurkat Line7: HepG2 Line8: NIH/3T3 Line9: Cos7

   

  

  Fig4: ICC staining EZR (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

   

  

  Fig6: Flow cytometric analysis of MCF-7 cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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