Anti-IL6 antibody [AH21-63]

Anti-IL6 antibody [AH21-63]

• 概述

• 产品描述Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.
• 产品名称Anti-IL6 antibody [AH21-63]
• 分子量24 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用ELISA(Cap),WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at -20 ℃. Stable for 12 months from date of receipt.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.20% Sodium Azide.
• 亚型IgG1
• 亚细胞定位Secreted.
• 其它名称
  • Interleukin BSF 2 antibody
  • B cell differentiation factor antibody
  • B cell stimulatory factor 2 antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  ELISA: 1:5,000-1:10,000

•  

  

  Fig1: Western blot analysis of IL6 on human spleen cell lysates using anti-IL6 antibody at 1/500 dilution.

   

  

  Fig2: ICC staining IL6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining IL6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining IL6 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL6 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL6 antibody. Counter stained with hematoxylin.

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