Anti-LRRN3 antibody [A4-6-3]

Anti-LRRN3 antibody [A4-6-3] 

• 概述

• 产品描述The LRRN3 is located on human chromosome 7, at 7q31.1.It contains 6 distinct gt-ag introns, and transcription produces five different mRNAs that appear to differ by truncation of the 3' end. There are only three main transcript variants that actually encode for the LRRN3 protein, with the longest transcript variant being 3744 base pairs in length. All three of these transcript variants have differing lengths and number of exons, but they all have the exon that includes the entire coding sequence for the LRRN3 protein.[8] There are also two paralogs to the LRRN3 gene. These include the LRRN1 and LRRN2 genes, both of which have the same leucine-rich repeats that are characteristic to this family of genes.
• 产品名称Anti-LRRN3 antibody [A4-6-3]
• 分子量79 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human LRRN3 aa 1-260.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Protein G affinity purified.
• 其它名称
  • Leucine-rich repeat neuronal protein 3L antibidy
  • Neuronal leucine-rich repeat protein 3 antibidy
  • NLRR-3 antibidy
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:100

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  Fig1: Western blot analysis of LRRN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: SHSY5Ycell lysate
  Lane 2: Rat testis tissue lysate

   

  

  Fig2: ICC staining of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody rature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

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