Anti-Claudin 1 antibody [8G1]

Anti-Claudin 1 antibody [8G1]

• 概述

• 产品描述The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-1 is a multi-pass membrane protein that is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Defects in the gene encoding claudin-1 are the cause of ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH), an autosomal recessive syndrome characterized by vulgar type ichthyosis, scalp hypotrichosis, scarring alopecia and sclerosing cholangitis.
• 产品名称Anti-Claudin 1 antibody [8G1]
• 分子量22 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 168-207 at the C-terminus of claudin-1 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell junction, tight junction. Cell membrane.
• 其它名称
  • Claudin-1 antibody
  • Claudin1 antibody
  • CLD 1 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

•  

  

  Fig1: Western blot analysis of claudin-1 expression in non-transfected 293T (A), human claudin-1 transfected 293T (B) and SCC-4 (C) whole cell lysates.

   

  

  Fig2: Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed,paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells (B). .

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