Anti-cytochrome C antibody [6G1]

Anti-cytochrome C antibody [6G1]

• 概述

• 产品描述Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
• 产品名称Anti-cytochrome C antibody [6G1]
• 分子量15kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IF,IP,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion intermembrane space
• 其它名称
  • CYC antibody
  • CYC_HUMAN antibody
  • CYCS antibody
  more

• 应用

  WB: 1:500-1:2,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500
  IHC-P: 1:50-500
  FC: 1 μg per 1 x 106 cells

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  Fig1: A.Western blot Western blot analysis of cytochrome c expression in HL-60 (A), K-562 (B) and PC-3 (C) whole cell lysates.

   

  

  Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tumor showing cytoplasmic staining (B).

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