Anti-Grp75 antibody [A6-A5-B1]

Anti-Grp75 antibody [A6-A5-B1]

• 概述

• 产品描述The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity
• 产品名称Anti-Grp75 antibody [A6-A5-B1]
• 分子量74kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Peptide affinity purified
• 亚细胞定位Mitochondrion, Nucleus
• 其它名称
  • 75 kDa glucose regulated protein antibody
  • 75 kDa glucose-regulated protein antibody
  • CSA antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis on different cell lysates using anti-Grp75 Mouse mAb. Positive control:
  Lane 1: HepG2
  Lane 2: L929
  Lane 3: Hela
  Lane 4: Jurkat

   

  

  Fig2: Immunocytochemical staining of Hela cells using anti-Grp75 Mouse mAb.

   

  

  Fig3: Immunocytochemical staining of PC-3M cells using anti-Grp75 Mouse mAb.

   

  

  Fig4: Immunocytochemical staining of MCF-7 cells using anti-Grp75 Mouse mAb.

   

  

  Fig5: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-Grp75 Mouse mAb.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-Grp75 Mouse mAb.

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-Grp75 Mouse mAb.

   

  

  Fig8: Flow cytometric analysis of HepG2 cells with Grp75 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary a

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