Anti-SCF antibody [4G5]

Anti-SCF antibody [4G5]

• 概述

• 产品描述Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger SCF form is expressed in fibroblasts, brain and thymus, while the smaller SCF form is found in the spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. With respect to mast cells, SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells
• 产品名称Anti-SCF antibody [4G5]
• 分子量48kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 26-214 of SCF of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Secreted, Cell membrane
• 其它名称
  • C kit ligand antibody
  • C-kit ligand antibody
  • Ckit ligand antibody
  more

• 应用

  WB: 1:200-1:2,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

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  Fig1: Western blot analysis of SCF expression in MCF7 (A) and COLO 320DM (B) whole cell lysates and rat brain (C), mouse brain (D), human colon (E) and human lung (F) tissue extracts.

   

  

  Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and extracellular staining of glandular cells (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of goblet cells (B).

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