Anti-GPX5 antibody [5A1]

Anti-GPX5 antibody [5A1]

• 概述

• 产品描述Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.
• 产品名称Anti-GPX5 antibody [5A1]
• 分子量22 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Peptide affinity purified
• 亚细胞定位Secreted. 
• 其它名称
  • Arep
  • arMEP24
  • EGLP
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  ICC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.

   

  

  Fig2: Western blot analysis of GPX5 on mouse testis tissue lysate using anti-GPX5 antibody at 1/500 dilution.

   

  

  Fig3: ICC staining GPX5 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining GPX5 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

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