Anti-LHCGR antibody [A2-G9]

Anti-LHCGR antibody [A2-G9]

• 概述

• 产品描述The luteinizing hormone/choriogonadotropin receptor (LHCGR), also lutropin/choriogonadotropin receptor (LCGR) or luteinizing hormone receptor (LHR) is a transmembrane receptor found predominantly in the ovary and testis, but also many extragonadal organs such as the uterus and breasts. The receptor interacts with both luteinizing hormone (LH) and chorionic gonadotropins (such as hCG in humans) and represents a G protein-coupled receptor (GPCR). Its activation is necessary for the hormonal functioning during reproduction.
• 产品名称Anti-LHCGR antibody [A2-G9]
• 分子量79 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • Gonadotropin receptor antibody
  • Gpcr19-rs1 antibody
  • GTHR-II antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of LHCGR on different lysates using anti-LHCGR antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: Jurkat
  Lane 3: SMMC-7721

   

  

  Fig2: ICC staining LHCGR (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Flow cytometric analysis of Jurkat cells with LHCGR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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