Anti-VIL1 antibody [G11-5E]

Anti-VIL1 antibody [G11-5E]

• 概述

• 产品描述Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.
• 产品名称Anti-VIL1 antibody [G11-5E]
• 分子量93 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, cytoskeleton.
• 其它名称
  • D2S1471 antibody
  • OTTHUMP00000164145 antibody
  • VIL antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Villin on human Villin recombinant protein using anti- Villin antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Villin on HEK293 (1) and Villin -hIgGFc transfected HEK293 (2) cell lysate using anti- Villin antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Villin on SW620 cell lysate using anti- Villin antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining Villin (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Villin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of SW620 cells with Villin antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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