Anti-HLA-B antibody [B10-G7]

Anti-HLA-B antibody [B10-G7]

• 概述

• 产品描述Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B and HLA-C encode membrane anchored heavy chains which heterodimerize with a light chain (β-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B and HLA-C alleles.
• 产品名称Anti-HLA-B antibody [B10-G7]
• 分子量41 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane
• 其它名称
  • AS antibody
  • HLA B protein antibody
  • HLA class I histocompatibility antigen, B alpha chain antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of HLA-B on Ramos(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！