Anti-ERN1 antibody [C9-F2]

Anti-ERN1 antibody [C9-F2]

• 概述

• 产品描述The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway.
• 产品名称Anti-ERN1 antibody [C9-F2]
• 分子量110 kDa
• 种属反应性Human
• 验证应用WB, IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Endoplasmic reticulum membrane.
• 其它名称
  • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
  • Endoplasmic reticulum to nucleus signaling 1 antibody
  • Endoplasmic reticulum-to-nucleus signaling 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of ERN1 on different cell lysate using anti-ERN1 antibody at 1/1,000 dilution.
  Positive control:
  Line1: Raji
  Line2: A431
  Line3: Jurkat
  Line4: Hela
  Line5: HEK293

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERN1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ERN1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ERN1 antibody. Counter stained with hematoxylin.

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