Anti-GUCY1A3 antibody [B2-G6]

Anti-GUCY1A3 antibody [B2-G6]

• 概述

• 产品描述Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one α subunit, designated GCS-α-1, and one β subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzyl -indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the α and β subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of α-1/β-1 and α-2/β-1 have been identified, and both display similar enzymatic activity.
• 产品名称Anti-GUCY1A3 antibody [B2-G6]
• 分子量77 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • GC S alpha 1 antibody
  • GC SA3 antibody
  • GCS alpha 1 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of GUCY1A3 on HEK293 (1) and GUCY1A3-hIgGFc transfected HEK293 (2) cell lysate using anti-GUCY1A3 antibody at 1/1,000

   

  

  Fig2: Western blot analysis of GUCY1A3 on different cell lysate using anti-GUCY1A3 antibody at 1/1,000 dilution.
  Positive control:
  Line1:HEK293 Line2:Raji

   

  

  Fig3: ICC staining GUCY1A3 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HEK293 cells with GUCY1A3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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